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Crystallization and preliminary X‐ray characterization of a type III cohesin–dockerin complex from the cellulosome system of

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In Ruminococcus flavefaciens, a predominant fibre‐degrading bacterium found in ruminants, cellulosomal proteins are anchored to the bacterial cell wall through a relatively small ScaE scaffoldin which includes a single type III cohesin. The cotton‐binding protein CttA consists of two cellulose‐binding modules and a C‐terminal modular pair (XDoc) comprising an X‐module and a contiguous dockerin, which exhibits high affinity towards the ScaE cohesin. Seleno‐l‐methionine‐labelled derivatives of the ScaE cohesin module and the XDoc from CttA have been expressed, copurified and cocrystallized. The crystals belonged to the tetragonal space group P43212, with unit‐cell parameters a = b = 78.7, c = 203.4 Å, and the unit cell contains a single cohesin–XDoc complex in the asymmetric unit. The diffraction data were phased to 2.0 Å resolution using the anomalous signal of the Se atoms.
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Document Type: Research Article

Publication date: September 1, 2012

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