Production and crystallization of α‐phosphoglucomutase from
α‐Phosphoglucomutase (α‐PGM) is an enzyme that is essential for the growth of Lactococcus lactis. The enzyme links bacterial anabolism with sugar utilization through glycolysis by catalyzing the reversible interconversion of glucose 6‐phosphate and α‐glucose 1‐phosphate. The gene encoding α‐PGM was cloned and overexpressed in L. lactis. The purified protein was functionally active and was crystallized with ammonium sulfate as a precipitant using vapour‐diffusion and seeding techniques. Optimized crystals diffracted to 1.5 Å resolution at a synchrotron source.
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Document Type: Research Article
Publication date: September 1, 2012