Purification, crystallization and preliminary X‐ray analysis of uracil‐DNA glycosylase from
Uracil‐DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N‐glycosidic bond, thereby initiating the base‐excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDGΔ) and stoUDGΔ complexed with uracil were crystallized and analyzed by X‐ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit‐cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDGΔ and stoUDGΔ complexed with uracil, respectively.
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Document Type: Research Article
Publication date: September 1, 2012