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Purification, crystallization and preliminary X‐ray analysis of uracil‐DNA glycosylase from

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Uracil‐DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N‐glycosidic bond, thereby initiating the base‐excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDGΔ) and stoUDGΔ complexed with uracil were crystallized and analyzed by X‐ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit‐cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDGΔ and stoUDGΔ complexed with uracil, respectively.
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Document Type: Research Article

Publication date: September 1, 2012

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