Crystallization and preliminary X‐ray crystallographic analysis of the putative NADP(H)‐dependent oxidoreductase YncB from
The yncB gene product from Vibrio vulnificus, which belongs to the medium‐chain dehydrogenase/reductase (MDR) superfamily, was crystallized using the microbatch crystallization method at 295 K. Diffraction data sets were collected using synchrotron radiation. Crystals of selenomethionine‐substituted YncB protein belonged to space group P212121, with unit‐cell parameters a = 90.52, b = 91.56, c = 104.79 Å. Assuming the presence of two molecules in the asymmetric unit, the solvent content was estimated to be about 57%. Crystals of the YncB–NADP(H) complex belonged to space group P41212 or P43212, with unit‐cell parameters a = b = 90.14, c = 105.61 Å. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 56.42%.
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Document Type: Research Article
Publication date: September 1, 2012