Skip to main content
padlock icon - secure page this page is secure

Expression, purification, crystallization and preliminary X‐ray analysis of a novel N‐substituted branched‐chain

Buy Article:

$52.00 + tax (Refund Policy)

Ferrous ion‐ and α‐ketoglutarate‐dependent dioxygenase from Burkholderia ambifaria AMMD (SadA) catalyzes the C3‐hydroxylation of N‐substituted branched‐chain l‐amino acids, especially N‐succinyl‐l‐leucine, coupled to the conversion of α‐ketoglutarate to succinate and CO2. SadA was expressed in Escherichia coli, purified and crystallized using the sitting‐drop vapour‐diffusion method at 293 K. Crystals of selenomethionine‐substituted SadA were obtained using a reservoir solution containing PEG 3000 as the precipitant at pH 9.5 and diffracted X‐rays to 2.4 Å resolution. The crystal belonged to space group P212121, with unit‐cell parameters a = 49.3, b = 70.9, c = 148.2 Å. The calculated Matthews coefficient (V M = 2.1 Å3 Da−1, 41% solvent content) suggested that the crystal contains two molecules per asymmetric unit.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: September 1, 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more