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Preliminary X‐ray crystallographic analysis of α‐carbonic anhydrase from

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Thiomicrospira crunogena XCL‐2 is a novel sulfur‐oxidizing chemolithoautotroph that plays a significant role in the sustainability of deep‐sea hydrothermal vent communities. This recently discovered gammaproteobacterium encodes and expresses four carbonic anhydrases (CAs) from three evolutionarily and structurally distinct CA families: an α‐CA, two β‐CAs and a γ‐CA. In order to characterize and elucidate the physiological roles of these CAs, X‐ray crystallographic structural studies have been initiated on the α‐CA. The α‐CA crystallized in space group C2. The crystals diffracted to a maximum resolution of 2.6 Å, with unit‐cell parameters a = 127.1, b = 102.2, c = 105.0 Å, β = 127.3°, and a calculated Matthews coefficient of 2.04 Å3 Da−1 with four identical protein molecules in the crystallographic asymmetric unit. A preliminary solution was determined by molecular replacement with the PHENIX AutoMR wizard, which had an initial TFZ score of 17.9. Refinement of the structure is currently in progress.
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Document Type: Research Article

Publication date: September 1, 2012

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