Skip to main content
padlock icon - secure page this page is secure

Cloning, expression, crystallization and preliminary X‐ray studies of the ferredoxin–NAD(P)

Buy Article:

$52.00 + tax (Refund Policy)

Ferredoxin–NADP+ reductase (FNR) is a flavoenzyme that catalyses the reduction of NADP+ in the final step of the photosynthetic electron‐transport chain. FNR from the thermophilic cyanobacterium Thermosynechococcus elongatus BP‐1 (TeFNR) contains an additional 9 kDa domain at its N‐terminus relative to chloroplastic FNRs and is more thermostable than those from mesophilic cyanobacteria. With the aim of understanding the structural basis of the thermostability of TeFNR and assigning a structural role to the small additional domain, the gene encoding TeFNR with and without an additional domain was engineered for heterologous expression and the recombinant proteins were purified and crystallized. Crystals of TeFNR without the additional domain belonged to space group P21, with unit‐cell parameters a = 55.05, b = 71.66, c = 89.73 Å, α = 90, β = 98.21, γ = 90°.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: September 1, 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more