Skip to main content
padlock icon - secure page this page is secure

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from

Buy Article:

$52.00 + tax (Refund Policy)

Dihydrodipicolinate synthase (DHDPS) catalyzes the first committed step of the lysine‐biosynthesis pathway in bacteria, plants and some fungi. This study describes the cloning, expression, purification and crystallization of DHDPS (NP_354047.1) from the plant pathogen Agrobacterium tumefaciens (AgT‐DHDPS). Enzyme‐kinetics studies demonstrate that AgT‐DHDPS possesses DHDPS activity in vitro. Crystals of AgT‐DHDPS were grown in the unliganded form and in forms with substrate bound and with substrate plus allosteric inhibitor (lysine) bound. X‐ray diffraction data sets were subsequently collected to a maximum resolution of 1.40 Å. Determination of the structure with and without substrate and inhibitor will offer insight into the design of novel pesticide agents.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: September 1, 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more