Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from
Dihydrodipicolinate synthase (DHDPS) catalyzes the first committed step of the lysine‐biosynthesis pathway in bacteria, plants and some fungi. This study describes the cloning, expression, purification and crystallization of DHDPS (NP_354047.1) from the plant pathogen Agrobacterium tumefaciens (AgT‐DHDPS). Enzyme‐kinetics studies demonstrate that AgT‐DHDPS possesses DHDPS activity in vitro. Crystals of AgT‐DHDPS were grown in the unliganded form and in forms with substrate bound and with substrate plus allosteric inhibitor (lysine) bound. X‐ray diffraction data sets were subsequently collected to a maximum resolution of 1.40 Å. Determination of the structure with and without substrate and inhibitor will offer insight into the design of novel pesticide agents.
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Document Type: Research Article
Publication date: September 1, 2012