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The mimivirus R355 gene product: preliminary crystallographic analysis of a putative ubiquitin‐like protein‐specific protease

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The complete genome sequence of the largest known double‐stranded DNA virus, mimivirus, reveals the presence of a gene (denoted R355) that potentially encodes a cysteine protease that is expressed late (after 6 h) in the infectious cycle of the virus. In order to verify a sequence‐based functional prediction and understand its role during the infectious process, the R355 protein was produced to assay its proteolytic activity and solve its three‐dimensional structure. Here, the preliminary crystallographic analysis of the recombinant viral protein is reported. The crystals belonged to the orthorhombic space group P212121, with a monomer in the asymmetric unit. A MAD data set was used for preliminary phasing using the selenium signal from a selenomethionine‐substituted protein crystal.
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Keywords: cysteine proteases; mimivirus; nucleocytoplasmic large DNA virus; polyprotein proteases; ubiquitin‐like protein‐specific protease

Document Type: Research Article

Affiliations: Information Génomique et Structurale, CNRS UPR 2589, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université, 163 Avenue de Luminy, Case 934, 13288 Marseille CEDEX 09, France

Publication date: January 1, 2011

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