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Crystallization and preliminary X‐ray analysis of a cold‐active endo‐β‐1,4‐d‐xylanase from glycoside hydrolase family 8

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Endo‐β‐1,4‐d‐xylanases are used in a multitude of industrial applications. Native crystals of a cold‐adapted xylanase from glycoside hydrolase family 8 were obtained by the vapour‐diffusion technique. The crystals belonged to space group I222, with unit‐cell parameters a = 46.6, b = 110.8, c = 150.2 Å at 100 K, and diffracted to 2.7 Å resolution at a synchrotron source. The asymmetric unit is likely to contain one molecule, with a V M of 2.07 Å3 Da−1, corresponding to a solvent content of ∼40%.
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Keywords: endo‐β‐1,4‐d‐xylanases; glycoside hydrolase family 8

Document Type: Research Article

Publication date: January 1, 2011

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