Skip to main content
padlock icon - secure page this page is secure

Expression, purification, crystallization and preliminary X‐ray analysis of the KaiC‐like protein PH0187 from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

Buy Article:

$52.00 + tax (Refund Policy)

KaiC is the central protein in the circadian rhythm in cyanobacteria. The 28 kDa KaiC‐like protein PH0187 from the hyperthermophilic archaeon Pyrococcus horikoshii was expressed in Escherichia coli, purified and crystallized using the sitting‐drop vapour‐diffusion method at 293 K. Crystals of PH0187 were obtained using a reservoir solution consisting of 1.0 M ammonium phosphate monobasic and 0.1 M sodium citrate tribasic pH 5.3 (the final pH value of the reservoir solution was 4.8) and diffracted X‐rays to 2.75 Å resolution. The crystal of PH0187 belonged to space group P6322, with unit‐cell parameters a = b = 239.1, c = 106.5 Å. The crystal contained four PH0187 molecules in the asymmetric unit.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: KaiC; PH0187; circadian rhythm

Document Type: Research Article

Affiliations: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

Publication date: January 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more