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Preliminary X‐ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species

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ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod‐shaped crystals belonged to space group P2122, with unit‐cell parameters a = 66.7, b = 131.7, c = 224.6 Å, α = 90, β = 90, γ = 90°. Data were collected to 2.9 Å resolution. A preliminary molecular‐replacement solution implied the presence of two ElaGT molecules in the asymmetric unit.
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Keywords: ElaGT; Streptomyces sp. SCSIO 01934; glycosyltransferases

Document Type: Research Article

Publication date: January 1, 2011

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