Crystallization and preliminary X‐ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94
PpAzoR, an FMN‐dependent NADPH azoreductase from Pseudomonas putida MET94, has been crystallized using the sitting‐drop vapour‐diffusion technique. The crystals diffracted to 1.6 Å resolution using synchrotron radiation and belonged to the orthorhombic space group F222, with unit‐cell parameters a = 72.1, b = 95.5, c = 146.1 Å. Data sets were collected from the native protein to 2.2 Å resolution using in‐house equipment and to 1.6 Å resolution using synchrotron radiation and the three‐dimensional structure was determined by the molecular‐replacement method.
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