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Crystallization and preliminary X‐ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94

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PpAzoR, an FMN‐dependent NADPH azoreductase from Pseudomonas putida MET94, has been crystallized using the sitting‐drop vapour‐diffusion technique. The crystals diffracted to 1.6 Å resolution using synchrotron radiation and belonged to the orthorhombic space group F222, with unit‐cell parameters a = 72.1, b = 95.5, c = 146.1 Å. Data sets were collected from the native protein to 2.2 Å resolution using in‐house equipment and to 1.6 Å resolution using synchrotron radiation and the three‐dimensional structure was determined by the molecular‐replacement method.
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Keywords: PpAzoR; Pseudomonas putida MET94; azoreductases

Document Type: Research Article

Affiliations: Instituto de Tecnologia Quimica e Biológica, Universidade Nova de Lisboa, Avenida da República, 2780-257 Oeiras, Portugal

Publication date: January 1, 2011

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