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Expression, purification and preliminary crystallographic analysis of the recombinant β‐glucosidase (BglA) from the halothermophile Halothermothrix orenii

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The β‐glucosidase A gene (bglA) has been cloned from the halothermophilic bacterium Halothermothrix orenii and the recombinant enzyme (BglA; EC 3.2.1.21) was bacterially expressed, purified using metal ion‐affinity chromatography and subsequently crystallized. Orthorhombic crystals were obtained that diffracted to a resolution limit of 3.5 Å. The crystal structure with two molecules in the asymmetric unit was solved by molecular replacement using a library of known glucosidase structures. Attempts to collect higher resolution diffraction data from crystals grown under different conditions and structure refinement are currently in progress.
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Keywords: BglA; Halothermothrix orenii; halothermophiles; β‐glucosidases

Document Type: Research Article

Publication date: January 1, 2011

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