Skip to main content
padlock icon - secure page this page is secure

Crystallization of an apo form of human arginase: using all the tools in the toolbox simultaneously

Buy Article:

$52.00 + tax (Refund Policy)

Arginase (EC 3.5.3.1) is an aminohydrolase that acts on l‐arginine to produce urea and ornithine. Two isotypes of the enzyme are found in humans. Type I is predominantly produced in the liver and is a homotrimer of 35 kDa subunits. Human arginase (hArginase) I is seen to be up‐regulated in many diseases and is a potential therapeutic target for many diverse indications. Previous reports of crystallization and structure determination of hArginase have always included inhibitors of the enzyme: here, the first case of a true apo crystal form of the enzyme which is suitable for small‐molecule soaking is reported. The crystals belonged to space group P212121 and have approximate unit‐cell parameters a = 53, b = 67.5, c = 250 Å. The crystals showed slightly anisotropic diffraction to beyond 2.0 Å resolution.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: alternative reservoirs; arginase; seeding

Document Type: Research Article

Publication date: January 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more