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Purification, crystallization and X‐ray diffraction study of basic 7S globulin from soybean

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Basic 7S globulin (Bg7S) is expressed by soybeans in response to biotic or abiotic stress. Bg7S is capable of binding to a 4 kDa protein which is supposedly involved in cell proliferation. Bg7S is widely found not only in legumes, but also in other plants; however, its function is still unclear. Here, Bg7S was successfully crystallized. Orthorhombic and monoclinic crystals of Bg7S were obtained under different conditions and belonged to space groups P21212, with unit‐cell parameters a = 111.9, b = 130.1, c = 287.8 Å, and P21, with unit‐cell parameters a = 85.3, b = 137.6, c = 162.1 Å, β = 91.2°, respectively.
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Keywords: basic 7S globulin; soybean

Document Type: Research Article

Publication date: January 1, 2011

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