Purification, crystallization and preliminary X‐ray diffraction of the G3BP1 NTF2‐like domain
The nuclear transport factor 2‐like (NTF2‐like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging‐drop vapour‐diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6322, with unit‐cell parameters a = b = 89.84, c = 70.02 Å. The crystals contained one molecule per asymmetric unit, with an estimated solvent content of 56%. Initial phases were obtained by molecular replacement.
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