Skip to main content
padlock icon - secure page this page is secure

Purification, crystallization and preliminary X‐ray diffraction of the G3BP1 NTF2‐like domain

Buy Article:

$52.00 + tax (Refund Policy)

The nuclear transport factor 2‐like (NTF2‐like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging‐drop vapour‐diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6322, with unit‐cell parameters a = b = 89.84, c = 70.02 Å. The crystals contained one molecule per asymmetric unit, with an estimated solvent content of 56%. Initial phases were obtained by molecular replacement.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: G3BP1; Ras signalling; RasGAP

Document Type: Research Article

Affiliations: Biostructural Research, Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark

Publication date: January 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more