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Crystallization and preliminary X‐ray studies of native and mutant intimin from enterohaemorrhagic Escherichia coli

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Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is a primarily food‐borne bacterial pathogen that is capable of causing life‐threatening human infections and poses a serious challenge to public health worldwide. The bacterial outer‐membrane protein intimin plays a key role in the initiation process of EHEC infection. In this study, intimin from EHEC O157:H7 (Int188) and its N916Y mutant (IntN916Y) were purified and crystals of both were obtained using the hanging‐drop vapour‐diffusion method at 291 K. Data were collected from Int188 and IntN916Y crystals to 2.8 and 2.6 Å resolution, respectively. The crystal of Int188 belonged to the orthorhombic space group C2, with unit‐cell parameters a = 235.16, b = 44.81, c = 129.12 Å, α = γ = 90, β = 97.53°. The crystal of IntN916Y belonged to space group P212121, with unit‐cell parameters a = 43.78, b = 92.49, c = 100.05 Å, α = β = γ = 90°.
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Keywords: EHEC O157:H7; Tir proteins; intimin

Document Type: Research Article

Publication date: January 1, 2011

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