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Crystallization of the Na+‐translocating NADH:quinone oxidoreductase from Vibrio cholerae

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The Na+‐translocating NADH:quinone oxidoreductase (Na+‐NQR) from the human pathogen Vibrio cholerae couples the exergonic oxidation of NADH by membrane‐bound quinone to Na+ translocation across the membrane. Na+‐NQR consists of six different subunits (NqrA–NqrF) and contains a [2Fe–2S] cluster, a noncovalently bound FAD, a noncovalently bound riboflavin, two covalently bound FMNs and potentially Q8 as cofactors. Initial crystallization of the entire Na+‐NQR complex was achieved by the sitting‐drop method using a nanolitre dispenser. Optimization of the crystallization conditions yielded flat yellow‐coloured crystals with dimensions of up to 200 × 80 × 20 µm. The crystals diffracted to 4.0 Å resolution and belonged to space group P21, with unit‐cell parameters a = 94, b = 146, c = 105 Å, α = γ = 90, β = 111°.
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Keywords: Na+‐NQR; Na+‐translocating NADH:quinone oxidoreductase; Vibrio cholerae; membrane‐protein complexes

Document Type: Research Article

Publication date: December 1, 2010

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