Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray diffraction analysis of a specific VHH domain against mouse prion protein

Buy Article:

$52.00 + tax (Refund Policy)

Prion disorders are infectious diseases that are characterized by the conversion of the cellular prion protein PrPC into the pathogenic isoform PrPSc. Specific antibodies that interact with the cellular prion protein have been shown to inhibit this transition. Recombinant VHHs (variable domain of dromedary heavy‐chain antibodies) or nanobodies are single‐domain antibodies, making them the smallest antigen‐binding fragments. A specific nanobody (Nb_PrP_01) was raised against mouse PrPC. A crystallization condition for this recombinant nanobody was identified using high‐throughput screening. The crystals were optimized using streak‐seeding and the hanging‐drop method. The crystals belonged to the orthorhombic space group P212121, with unit‐cell parameters a = 30.04, b = 37.15, c = 83.00 Å, and diffracted to 1.23 Å resolution using synchrotron radiation. The crystal structure of this specific nanobody against PrPC together with the known PrPC structure may help in understanding the PrPC/PrPSc transition mechanism.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Camelidae; PrPC; PrPSc; nanobodies; prions

Document Type: Research Article

Publication date: December 1, 2010

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more