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Crystallographic studies of the coupling segment NBD94674–781 of the nucleotide‐binding domain of the Plasmodium yoelii reticulocyte‐binding protein Py235

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The Plasmodium yoelii reticulocyte‐binding protein Py235 has a role as an ATP/ADP sensor. The sensor domain of Py235 is called NBD94; it consists of at least three functional regions, the nucleotide‐binding region (NBD94444–547), hinge region (NBD94566–663) and C‐terminal coupling region (NBD94674–781), and has been proposed to link ATP/ADP binding to the interaction of Py235 with the red blood cell. Here, NBD94674–781 was cloned, expressed and purified to high purity. The monodisperse protein was crystallized by vapour diffusion. A diffraction data set was collected to 2.9 Å resolution with 97.2% completeness using a synchrotron‐radiation source. The crystals belonged to space group C2, with unit‐cell parameters a = 65.08, b = 82.71, c = 114.27 Å, β = 94.72°, and contained four molecules in the asymmetric unit.
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Keywords: ATP/ADP sensors; Plasmodium; malaria; nucleotide‐binding domain

Document Type: Research Article

Affiliations: Nanyang Technological University, School of Biological Sciences, 60 Nanyang Drive, Singapore 637551, Singapore

Publication date: December 1, 2010

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