Expression, purification, crystallization and initial X‐ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis
Thiol peroxidase is an atypical 2‐Cys peroxiredoxin that reduces alkyl hydroperoxides. Wild‐type and C61S mutant protein have been recombinantly expressed in Escherichia coli and purified using nickel‐affinity chromatography. Initial crystallization trials yielded three crystal forms in three different space groups (P21, P64 and P212121) both in the presence and the absence of DTT.
No Supplementary Data
No Article Media