Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray analysis of SDR‐type pyridoxal dehydrogenase from Mesorhizobium loti

Buy Article:

$52.00 + tax (Refund Policy)

Pyridoxal 4‐dehydrogenase from Mesorhizobium loti MAFF303099 was overexpressed in Escherichia coli. The recombinant selenomethionine‐substituted enzyme was purified and crystallized by the sitting‐drop vapour‐diffusion method using PEG 4000 as precipitant. Crystals grew in the presence of 0.45 mM NAD+. The crystals diffracted to 2.9 Å resolution and belonged to the monoclinic space group P21, with unit‐cell parameters a = 86.20, b = 51.11, c = 91.73 Å, β = 89.36°. The calculated V M values suggested that the asymmetric unit contained four molecules.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Mesorhizobium loti; SDR‐type pyridoxal 4‐dehydrogenase; pyridoxine‐degradation pathway

Document Type: Research Article

Publication date: June 1, 2010

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more