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Crystallization and preliminary X‐ray analysis of SDR‐type pyridoxal dehydrogenase from Mesorhizobium loti

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Pyridoxal 4‐dehydrogenase from Mesorhizobium loti MAFF303099 was overexpressed in Escherichia coli. The recombinant selenomethionine‐substituted enzyme was purified and crystallized by the sitting‐drop vapour‐diffusion method using PEG 4000 as precipitant. Crystals grew in the presence of 0.45 mM NAD+. The crystals diffracted to 2.9 Å resolution and belonged to the monoclinic space group P21, with unit‐cell parameters a = 86.20, b = 51.11, c = 91.73 Å, β = 89.36°. The calculated V M values suggested that the asymmetric unit contained four molecules.
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Keywords: Mesorhizobium loti; SDR‐type pyridoxal 4‐dehydrogenase; pyridoxine‐degradation pathway

Document Type: Research Article

Publication date: June 1, 2010

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