Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray diffraction studies of a ferredoxin reductase component of carbazole 1,9a‐dioxygenase from Novosphingobium sp. KA1

Buy Article:

$52.00 + tax (Refund Policy)

Carbazole 1,9a‐dioxygenase (CARDO) is the initial enzyme of the carbazole‐degradation pathway. The CARDO of Novosphingobium sp. KA1 consists of a terminal oxygenase, a putidaredoxin‐type ferredoxin and a ferredoxin‐NADH oxidoreductase (Red) and is classified as a class IIA Rieske oxygenase. Red from KA1 was crystallized at 278 K by the hanging‐drop vapour‐diffusion method using PEG 4000. The crystal diffracted to 1.58 Å resolution and belonged to space group P32, with unit‐cell parameters a = b = 92.2, c = 78.6 Å, α = γ = 90, β = 120°. Preliminary analysis of the X‐ray diffraction data revealed that the asymmetric unit contained two Red monomers. The crystal appeared to be a merohedral twin, with a twin fraction of 0.32 and twin law (−h, −kl).
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Rieske nonhaem iron oxygenases; carbazole; ferredoxin reductases

Document Type: Research Article

Publication date: June 1, 2010

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more