Crystallization and preliminary X‐ray diffraction studies of a ferredoxin reductase component of carbazole 1,9a‐dioxygenase from Novosphingobium sp. KA1
Carbazole 1,9a‐dioxygenase (CARDO) is the initial enzyme of the carbazole‐degradation pathway. The CARDO of Novosphingobium sp. KA1 consists of a terminal oxygenase, a putidaredoxin‐type ferredoxin and a ferredoxin‐NADH oxidoreductase (Red) and is classified as a class IIA Rieske oxygenase. Red from KA1 was crystallized at 278 K by the hanging‐drop vapour‐diffusion method using PEG 4000. The crystal diffracted to 1.58 Å resolution and belonged to space group P32, with unit‐cell parameters a = b = 92.2, c = 78.6 Å, α = γ = 90, β = 120°. Preliminary analysis of the X‐ray diffraction data revealed that the asymmetric unit contained two Red monomers. The crystal appeared to be a merohedral twin, with a twin fraction of 0.32 and twin law (−h, −k, l).
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