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Crystallization and preliminary X‐ray crystallographic studies of a Lys49‐phospholipase A2 homologue from Bothrops pirajai venom complexed with rosmarinic acid

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PrTX‐I, a noncatalytic and myotoxic Lys49‐phospholipase A2 from Bothrops pirajai venom, was crystallized in the presence of the inhibitor rosmarinic acid (RA). This is the active compound in the methanolic extract of Cordia verbenacea, a plant that is largely used in Brazilian folk medicine. The crystals diffracted X‐rays to 1.8 Å resolution and the structure was solved by molecular‐replacement techniques, showing electron density that corresponds to RA molecules at the entrance to the hydrophobic channel. The crystals belong to space group P212121, indicating conformational changes in the structure after ligand binding: the crystals of all apo Lys49‐phospholipase A2 structures belong to space group P3121, while the crystals of complexed structures belong to space groups P21 or P212121.
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Keywords: Bothrops pirajai venom; Cordia verbenacea; myotoxins; phospholipase A2; rosmarinic acid

Document Type: Research Article

Publication date: June 1, 2010

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