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Crystallization and preliminary crystallographic studies of CorC, a magnesium‐ion transporter

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CorC is a magnesium transporter that is involved in the Mg2+‐efflux function of the CorA transporter system, an Mg2+ channel, from Shigella flexneri. Native CorC was purified and crystallized in the native form and in a ligand‐free form and diffraction data sets were collected to 2.9 and 3.4 Å resolution, respectively. The native CorC crystals belonged to space group P212121, with unit‐cell parameters a = 64.31, b = 74.44, c = 132.78 Å. The ligand‐free CorC crystals belonged to space group P3121/P3221, with unit‐cell parameters a = b = 71.89, c = 125.96 Å. The CorC–ATP complex has also been crystallized and the crystals belonged to space group P2 or P21.
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Keywords: CorA transporter system; CorC; Shigella flexneri; magnesium‐ion transporters

Document Type: Research Article

Publication date: June 1, 2010

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