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Crystallization and preliminary crystallographic analysis of N‐acetyltransferase Mpr1 from Saccharomyces cerevisiae

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Mpr1 is an enzyme that catalyzes the N‐acetylation of the toxic l‐azetidine‐2‐carboxylic acid (AZC). Recently, Mpr1 has been shown to reduce levels of intracellular reactive oxygen species (ROS) under oxidative stress. The natural substrate involved in the ROS elimination in vivo is still unknown. Mpr1 has been purified and crystallized in space groups P1 and P3112. X‐ray data were collected to 1.9 Å resolution from a trigonal crystal soaked with AZC.
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Keywords: N‐acetyltransferases; oxidative stress; proline metabolism

Document Type: Research Article

Publication date: February 1, 2009

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