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Crystallization and preliminary X‐ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

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Alanine racemase (DadXOF4), a dimeric endogenous PLP‐dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His6 tag in a form suitable for X‐ray crystallographic analysis. Crystals were grown by the hanging‐drop vapour‐diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P212121, with two protein molecules in the asymmetric unit.
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Keywords: Bacillus pseudofirmus OF4; alkaline alanine racemase

Document Type: Research Article

Publication date: February 1, 2009

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