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Crystallization and diffraction of an Lhx4–Isl2 complex

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A stable intramolecular complex comprising the LIM domains of the LIM‐homeodomain protein Lhx4 tethered to a peptide region of Isl2 has been engineered, purified and crystallized. The monoclinic crystals belonged to space group P21, with unit‐cell parameters a = 46.8, b = 88.7, c = 49.9 Å, β = 111.9°, and diffracted to 2.16 Å resolution.
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Keywords: Isl2; Lhx4; Lim domains; Lim‐homeodomain transcription factors

Document Type: Research Article

Affiliations: School of Molecular and Microbial Biosciences, University of Sydney, Australia

Publication date: February 1, 2009

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