Crystallization and preliminary X‐ray analysis of 2,3‐diketo‐5‐methylthiopentyl‐1‐phosphate enolase from Bacillus subtilis
2,3‐Diketo‐5‐methylthiopentyl‐1‐phosphate enolase (DK‐MTP‐1P enolase) from Bacillus subtilis was crystallized using the hanging‐drop vapour‐diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 Å resolution at 100 K using synchrotron radiation and were found to belong to the monoclinic space group P21, with unit‐cell parameters a = 79.3, b = 91.5, c = 107.0 Å, β = 90.8°. The asymmetric unit contained four molecules of DK‐MTP‐1P enolase, with a V M value of 2.2 Å3 Da−1 and a solvent content of 43%.
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