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Crystallization and preliminary X‐ray analysis of 2,3‐diketo‐5‐methylthiopentyl‐1‐phosphate enolase from Bacillus subtilis

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2,3‐Diketo‐5‐methylthiopentyl‐1‐phosphate enolase (DK‐MTP‐1P enolase) from Bacillus subtilis was crystallized using the hanging‐drop vapour‐diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 Å resolution at 100 K using synchrotron radiation and were found to belong to the monoclinic space group P21, with unit‐cell parameters a = 79.3, b = 91.5, c = 107.0 Å, β = 90.8°. The asymmetric unit contained four molecules of DK‐MTP‐1P enolase, with a V M value of 2.2 Å3 Da−1 and a solvent content of 43%.
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Keywords: 2,3‐diketo‐5‐methylthiopentyl‐1‐phosphate enolase; Bacillus subtilis; RuBisCO; RuBisCO‐like proteins; methionine‐salvage pathway

Document Type: Research Article

Publication date: February 1, 2009

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