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Crystallization and X‐ray diffraction analysis of the RNA primer/promoter‐binding domain of influenza A virus RNA‐dependent RNA polymerase PB2

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The C‐terminal domain protein (amino‐acid residues 535–759) of the PB2 subunit of the RNA‐dependent RNA polymerase from the highly pathogenic influenza A virus was expressed as a soluble protein in Escherichia coli and crystallized using sodium formate as a precipitant. Data sets were collected from crystals of native and selenomethionine‐substituted protein on the KEK NW12 beamline at the Photon Factory and the crystals diffracted to a maximum resolution of 2.44 Å for the SeMet‐derivative crystal. The native crystals were found to belong to space group P3221, with unit‐cell parameters a = b = 52.5, c = 156.3 Å. The Matthews value (V M) was 2.7 Å3 Da−1, assuming the presence of one molecule in the asymmetric unit. The SeMet‐derivative crystals were found to belong to the same space group, with unit‐cell parameters a = b = 52.6, c = 156.4 Å. Attempts are being made to solve the structure by multi‐wavelength anomalous dispersion phasing.
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Keywords: PB2; RNA‐dependent RNA polymerase; influenza A virus

Document Type: Research Article

Publication date: February 1, 2009

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