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Expression, purification, crystallization and preliminary X‐ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes

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The soluble domain (residues 483–913) of PPA0092, a putative copper‐containing nitrite reductase from Propionibacterium acnes KPA171202, has been overexpressed in Escherichia coli. The purified recombinant protein was crystallized using the hanging‐drop vapour‐diffusion method. X‐ray diffraction data were collected and processed to a maximum resolution of 2.4 Å. The crystal belonged to space group P213, with unit‐cell parameters a = b = c = 108.63 Å. Preliminary diffraction data show that one molecule is present in the asymmetric unit; this corresponds to a V M of 2.1 Å3 Da−1.
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Keywords: acne; copper; denitrification; nitrite reductases

Document Type: Research Article

Affiliations: Bioinorganic Chemistry Laboratory, Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

Publication date: February 1, 2009

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