@article {Hira:2009:1744-3091:116,
title = "Crystallization and preliminary Xray diffraction analysis of a complex between the electrontransfer partners hexameric Cucontaining nitrite reductase and pseudoazurin",
journal = "Acta Crystallographica Section F",
parent_itemid = "infobike://bpl/ayf2",
publishercode ="bp",
year = "2009",
volume = "65",
number = "2",
publication date ="2009-02-01T00:00:00",
pages = "116-119",
itemtype = "ARTICLE",
issn = "1744-3091",
url = "https://www.ingentaconnect.com/content/bpl/ayf2/2009/00000065/00000002/art00012",
doi = "doi:10.1107/S1744309108040219",
keyword = "Cu‐containing nitrite reductases, pseudoazurins, electron‐transfer complexes",
author = "Hira, Daisuke and Nojiri, Masaki and Suzuki, Shinnichiro",
abstract = "The complex between Cucontaining nitrite reductase (HdNIR) and its electrondonor protein pseudoazurin (HdPAz) from Hyphomicrobium denitrificans has been crystallized. The crystals were obtained from a mixture of the two proteins using the hangingdrop vapourdiffusion
method in the presence of polyethylene glycol (PEG) and 2methyl2,4pentanediol (MPD) as precipitants. SDSPAGE analysis demonstrated that the crystals contained both proteins. The Xray diffraction experiment was carried out at SPring8 and diffraction
data were collected to 3.3\AA resolution. The crystals were tetragonal (space group P41212), with unitcell parameters a = b = 130.39, c = 505.55\AA. Preliminary analysis indicated that there was one HdNIR and
at least two HdPAz molecules in the asymmetric unit of the crystal.",
}