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Crystallization and preliminary X‐ray diffraction analysis of human IL‐22 bound to its soluble decoy receptor IL‐22BP

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Interleukin‐22 (IL‐22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL‐22 was incubated with its soluble decoy receptor IL‐22BP (IL‐22 binding protein) and the IL‐22–IL‐22BP complex was crystallized in hanging drops using the vapour‐diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 Å resolution. The crystal belonged to the tetragonal space group P41, with unit‐cell parameters a = b = 67.9, c = 172.5 Å, and contained two IL‐22–IL‐22BP complexes per asymmetric unit.
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Keywords: IL‐22; IL‐22BP; cytokines; interleukins

Document Type: Research Article

Publication date: February 1, 2009

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