Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2)
A non‐Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting‐drop vapour‐diffusion method. An X‐ray diffraction data set was collected to 2.0 Å resolution. The crystal belonged to space group P21, with unit‐cell parameters a = 29.67, b = 67.00, c = 34.43 Å, α = γ = 90.00, β = 94.26°.
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