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Cloning, expression, purification, crystallization and preliminary X‐ray analysis of the human RuvBL1–RuvBL2 complex

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The complex of RuvBL1 and its homologue RuvBL2, two evolutionarily highly conserved eukaryotic proteins belonging to the AAA+ (ATPase associated with diverse cellular activities) family of ATPases, was co‐expressed in Escherichia coli. For crystallization purposes, the flexible domains II of RuvBL1 and RuvBL2 were truncated. The truncated RuvBL1–RuvBL2 complex was crystallized using the hanging‐drop vapour‐diffusion method at 293 K. The crystals were hexagonal‐shaped plates and belonged to either the orthorhombic space group C2221, with unit‐cell parameters a = 111.4, b = 188.0, c = 243.4 Å and six monomers in the asymmetric unit, or the monoclinic space group P21, with unit‐cell parameters a = 109.2, b = 243.4, c = 109.3 Å, β = 118.7° and 12 monomers in the asymmetric unit. The crystal structure could be solved by molecular replacement in both possible space groups and the solutions obtained showed that the complex forms a dodecamer.
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Keywords: ATPases; RuvBL1; RuvBL2

Document Type: Research Article

Publication date: September 1, 2008

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