Purification, crystallization and preliminary X‐ray diffraction analysis of a cystathionine β‐synthase domain‐containing protein, CDCP2, from Arabidopsis thaliana
Cystathione β‐synthase domain‐containing protein 2 (CDCP2) from Arabidopsis thaliana has been overexpressed and purified to homogeneity. As an initial step towards three‐dimensional structure determination, crystals of recombinant CDCP2 protein have been obtained using polyethylene glycol 8000 as a precipitant. The crystals diffracted to 2.4 Å resolution using synchrotron radiation and belonged to the trigonal space group P3121 or P3221, with unit‐cell parameters a = b = 56.360, c = 82.596 Å, α = β = 90, γ = 120°. The asymmetric unit contains one CDCP2 molecule and the solvent content is approximately 41%.
No Supplementary Data
No Article Media