Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ
Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His‐tagged p50 subunit (p50) and a p50‐interacting domain of the p66 subunit (p66N) was crystallized. The crystal was in the form of a prism with a rhombic cross‐section and belonged to space group P21. The crystal had unit‐cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50–p66N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.
No Supplementary Data
No Article Media
Document Type: Research Article
Affiliations: Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-7696, USA
Publication date: September 1, 2008