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Crystallographic study of G178S mutant of human proliferating cell nuclear antigen

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Proliferating cell nuclear antigen (PCNA) is an evolutionarily conserved protein that forms a ring‐shaped homotrimer that functions as a sliding clamp for DNA replication. The rev6‐1 mutation of Saccharomyces cerevisiae, which inactivates both translesion DNA synthesis and damage‐avoidance pathways while having little effect on normal cell growth, has a G178S substitution in the PCNA protein. Human PCNA protein carrying the G178S substitution was crystallized. Two crystal forms were obtained under similar conditions. Crystal forms I and II belong to space groups P21, with unit‐cell parameters a = 84.1, b = 130.2, c = 97.8 Å, β = 113.4°, and P212121, with unit‐cell parameters a = 68.1, b = 100.2, c = 131.2 Å, respectively. Structural analyses by molecular replacement are now in progress.
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Keywords: DNA damage tolerance; DNA replication; PCNA; translesion DNA synthesis

Document Type: Research Article

Publication date: September 1, 2008

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