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Expression, purification and preliminary crystallographic analysis of N‐acetylglucosamine‐1‐phosphate uridylyltransferase from Mycobacterium tuberculosis

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The gene product of open reading frame Rv1018c from Mycobacterium tuberculosis is annotated as encoding a probable N‐acetylglucosamine 1‐phosphate uridylyltransferase (MtbGlmU), an enzyme that catalyzes the biosynthesis of UDP‐N‐acetylglucosamine, a precursor common to lipopolysaccharide and peptidoglycan biosynthesis. Following overexpression in Escherichia coli, the enzyme was purified and crystallized using the hanging‐drop vapour‐diffusion method. Native diffraction data were collected from crystals belonging to space group R32 and processed to a resolution of 2.2 Å.
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Keywords: GlmU; Mycobacterium tuberculosis H37Rv; N‐acetylglucosamine 1‐phosphate uridyltransferase; Rv1018c; peptidoglycan metabolism

Document Type: Research Article

Affiliations: Protein Structure and Function Group, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Publication date: September 1, 2008

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