Crystallization and preliminary crystallographic analysis of the ferredoxin component of carbazole 1,9a‐dioxygenase from Nocardioides aromaticivorans IC177
Carbazole 1,9a‐dioxygenase (CARDO) catalyzes the dihydroxylation of carbazole by angular position (C9a) carbon bonding to the imino nitrogen and its adjacent C1 carbon. CARDO consists of a terminal oxygenase component and two electron‐transfer components: ferredoxin and ferredoxin reductase. The ferredoxin component of carbazole 1,9a‐dioxygenase from Nocardioides aromaticivorans IC177 was crystallized at 293 K using the hanging‐drop vapour‐diffusion method with ammonium sulfate as the precipitant. The crystals, which were improved by macroseeding, diffract to 2.0 Å resolution and belong to space group P41212.
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