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Cocrystallizing natural RNA with its unnatural mirror image: biochemical and preliminary X‐ray diffraction analysis of a 5S rRNA A‐helix racemate

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Chemically synthesized RNAs with the unnatural l‐configuration possess enhanced in vivo stability and nuclease resistance, which is a highly desirable property for pharmacological applications. For a structural comparison, both l‐ and d‐RNA oligonucleotides of a shortened Thermus flavus 5S rRNA A‐helix were chemically synthesized. The enantiomeric RNA duplexes were stochiometrically cocrystallized as a racemate, which enabled analysis of the d‐ and l‐RNA enantiomers in the same crystals. In addition to a biochemical investigation, diffraction data were collected to 3.0 Å resolution using synchrotron radiation. The crystals belonged to space group P3121, with unit‐cell parameters a = b = 35.59, c = 135.30 Å, γ = 120° and two molecules per asymmetric unit.
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Keywords: 5S rRNA; RNA racemates; d‐ and l‐RNA enantiomers; microhelices; oligonucleotides; spiegelmers

Document Type: Research Article

Publication date: October 1, 2007

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