Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray diffraction analysis of the full‐length Mg2+ transporter MgtE

Buy Article:

$52.00 + tax (Refund Policy)

The MgtE family of Mg2+ transporters are ubiquitously conserved in all three domains. The genes encoding full‐length MgtE from seven different species were cloned. Three of the seven MgtE transporters were overexpressed and purified for use in crystallization trials. Only Thermus thermophilus MgtE was successfully crystallized using the sitting‐drop vapour‐diffusion method. Selenomethionine‐substituted (SeMet) crystals were obtained by cross‐microseeding using the native microcrystals. The SeMet crystals diffracted X‐rays to 3.5 Å resolution using synchrotron radiation and belong to space group C2221, with unit‐cell parameters a = 118.3, b = 134.9, c = 366.2 Å. Structure determination is in progress.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: CBS domain; MgtE; lipids; magnesium; transporter

Document Type: Research Article

Publication date: August 1, 2007

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more