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Crystallization and preliminary X‐ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE

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The MgtE family of Mg2+ transporters is ubiquitously conserved in all domains of life. The cytosolic domains of the MgtE Mg2+ transporters include a cystathionine‐β‐synthase (CBS) domain which is known to play a regulatory function in transporter proteins. The cytosolic domain of MgtE from Thermus thermophilus was overexpressed, purified and crystallized in the presence and absence of Mg2+. The crystals formed in the presence of Mg2+ diffracted X‐rays to 2.3 Å resolution using synchrotron radiation, belong to space group P6522 with unit‐cell parameters a = b = 57.7, c = 317.6 Å and are expected to contain one molecule in the asymmetric unit. The crystals formed in the absence of Mg2+ diffracted X‐rays to 3.5 Å resolution using synchrotron radiation, belong to space group P212121 with unit‐cell parameters a = 77.0, b = 100.3, c = 100.3 Å and are expected to contain two molecules in the asymmetric unit.
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Keywords: CBS domain; MgtE; cytosolic domain; magnesium; transporter

Document Type: Research Article

Publication date: August 1, 2007

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