Purification, crystallization and preliminary X‐ray diffraction studies of Rab11 in complex with Rab11‐FIP2
The small GTPase Rab11 regulates the recycling of endosomes back to the plasma membrane. In its active GTP‐bound form, Rab11 binds a novel set of effectors termed the Rab11 family of interacting proteins (Rab11‐FIPs) which contain a conserved C‐terminal Rab‐binding domain (RBD) of unknown structure. Here, a complex of Rab11 with the RBD of Rab11‐FIP2 has been purified and crystallized in the trigonal space group P3121, with unit‐cell parameters a = 64.99, b = 64.99, c = 112.59 Å. Static light‐scattering analyses of the molecular weight of the complex in solution are consistent with two copies of Rab11 and two copies of Rab11‐FIP2 in the complex.
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