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Crystallization and preliminary X‐ray diffraction analysis of an anti‐H(O) lectin from Lotus tetragonolobus seeds

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The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour‐diffusion method at a constant temperature of 293 K. A complete structural data set was collected at 2.00 Å resolution using a synchrotron‐radiation source. LTA crystals were found to be monoclinic, belonging to space group P21, with unit‐cell parameters a = 68.89, b = 65.83, c = 102.53 Å, α = γ = 90, β = 92°. Molecular replacement yielded a solution with a correlation coefficient and R factor of 34.4 and 51.6%, respectively. Preliminary analysis of the molecular‐replacement solution indicates a new quaternary association in the LTA structure. Crystallographic refinement is under way.
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Keywords: LTA; Lotus tetragonolobus

Document Type: Research Article

Publication date: July 1, 2006

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