Crystallization and preliminary X‐ray diffraction analysis of an anti‐H(O) lectin from Lotus tetragonolobus seeds
The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour‐diffusion method at a constant temperature of 293 K. A complete structural data set was collected at 2.00 Å resolution using a synchrotron‐radiation source. LTA crystals were found to be monoclinic, belonging to space group P21, with unit‐cell parameters a = 68.89, b = 65.83, c = 102.53 Å, α = γ = 90, β = 92°. Molecular replacement yielded a solution with a correlation coefficient and R factor of 34.4 and 51.6%, respectively. Preliminary analysis of the molecular‐replacement solution indicates a new quaternary association in the LTA structure. Crystallographic refinement is under way.
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