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Comparative crystallization and preliminary X‐ray diffraction studies of locked nucleic acid and RNA stems of a tenascin C‐binding aptamer

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The pharmacokinetic properties of an aptamer against the tumour‐marker protein tenascin‐C have recently been successfully improved by the introduction of locked nucleic acids (LNAs) into the terminal stem of the aptamer. Since it is believed that this post‐SELEX optimization is likely to provide a more general route to enhance the in vitro and in vivo stability of aptamers, elucidation of the structural basis of this improvement was embarked upon. Here, the crystallographic and X‐ray diffraction data of the isolated aptamer stem encompassed in a six‐base‐pair duplex both with and without the LNA modification are presented. The obtained all‐LNA crystals belong to space group P41212 or P43212, with unit‐cell parameters a = b = 52.80, c = 62.83 Å; the all‐RNA crystals belong to space group R32, with unit‐cell parameters a = b = 45.21, c = 186.97 Å, γ = 120.00°.
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Keywords: LNA; TTA1; aptamer; locked nucleic acids; tenascin‐C

Document Type: Research Article

Publication date: July 1, 2006

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