Skip to main content
padlock icon - secure page this page is secure

Cloning, expression, purification and preliminary crystallographic characterization of a shikimate dehydrogenase from Corynebacterium glutamicum

Buy Article:

$47.00 + tax (Refund Policy)

The shikimate dehydrogenase from Corynebacterium glutamicum has been cloned into an Escherichia coli expression vector, overexpressed and purified. Native crystals were obtained by the vapour‐diffusion technique using 2‐methyl‐2,4‐pentanediol as a precipitant. The crystals belong to the centred monoclinic space group C2, with unit‐cell parameters a = 118.77, b = 63.17, c = 35.67 Å, β = 92.26° (at 100 K), and diffract to 1.64 Å on a synchrotron X‐ray source. The asymmetric unit is likely to contain one molecule, corresponding to a packing density of 2.08 Å3 Da−1 and a solvent content of about 41%.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Corynebacterium glutamicum; shikimate dehydrogenase

Document Type: Research Article

Affiliations: Institute for Biochemistry, University of Köln, Zülpicher Strasse 47, Köln, NRW 50974, Germany

Publication date: July 1, 2006

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more