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Crystallization and preliminary crystallographic analysis of the site‐specific DNA nickase Nb.BspD6I

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Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.
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Keywords: Bacillus sp. D6; Nb.BspD6I; site‐specific DNA nickase

Document Type: Research Article

Publication date: March 1, 2005

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