Skip to main content
padlock icon - secure page this page is secure

Energetics of 5‐bromo‐4‐chloro‐3‐indolyl‐α‐d‐mannose binding to the Parkia platycephala seed lectin and its use for MAD phasing

Buy Article:

$52.00 + tax (Refund Policy)

Parkia platycephala belongs to the most primitive group of Leguminosae plants. Its seed lectin is made up of three homologous β‐prism repeats and exhibits binding specificity for mannose/glucose. The properties of the association between the lectin from P. platycephala seeds and monosaccharide ligands were analysed by isothermal titration calorimetry and surface plasmon resonance. The results are consistent with the lectin bearing three thermodynamically identical binding sites for mannose/glucose per monomer with dissociation constants in the millimolar range. Binding of each ligand by the lectin is enthalpically driven. Crystals have been obtained of the lectin in complex with a brominated derivative of mannose (5‐bromo‐4‐chloro‐3‐indolyl‐α‐d‐mannose), which were suitable for deriving an electron‐density map by MAD phasing. In agreement with the thermodynamic data, six Br atoms were found in the asymmetric unit of the monoclinic P21 crystals, which contained two P. platycephala lectin molecules. The availability of other Br derivatives of monosaccharides (glucose, galactose, fucose) may make this strategy widely useful for structure elucidation of novel lectins or when (as in the case of the P. platycephala lectin) molecular‐replacement methods fail.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: MAD phasing; Parkia platycephala lectin; isothermal titration calorimetry; protein–carbohydrate interactions; surface plasmon resonance; β‐prism domain

Document Type: Research Article

Publication date: March 1, 2005

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more